Fig. 7

Validation of hsa-miR-302b-3p target genes in human cardiac fibroblasts (hCFs) upon EV-H5 (from hiPS-L3) treatment with the real-time qPCR method. hCFs were transfected with specific miRNA inhibitors or miRNA mimic and treated with EV-H5 and TGFβ (1 ng/ml). Mock-transfected and TGFβ-treated cells were used as control (CTRL). Targets for miR-302b-3p were selected using the TargetScanHuman 8.0, the miRDB, and the DIANA mirPath v.3 databases. Abbreviations: C-i, miRNA-non-targeting inhibitor; miR302-i, miR-302b-3p inhibitor; miR302-m, miR-302b-3p mimic. All data are presented as the mean ± SD (n = 3). Statistical significance was tested using one-way ANOVA and the Tukey post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001