Table 1 List of antibodies used in the study
Antibody | Specification | Assay |
|---|---|---|
Primary antibodies | ||
Anti-OCT4 | Monoclonal mouse, IgG, Invitrogen #MA1-104 | IF (1:200), WB (1:1000) |
Anti-SSEA4 | Mouse, IgG3, Invitrogen #A24866 | IF (1:100) |
Anti-CD133 | Monoclonal mouse, IgG1, Thermo Fisher Scientific #MA1-219 | IF (1:200), WB (1:1000) |
Anti-α-SMA | Monoclonal mouse, Sigma-Aldrich #A2547 | IF (1:400), WB (1:1000) |
Anti-Vinculin | Monoclonal mouse, Sigma-Aldrich #V9264 | IF (1:200), WB (1:1000) |
Anti-pSmad2 | Polyclonal rabbit, Cell Signaling Technology #3108 | IF (1:100) |
Anti-Slug2 | Polyclonal rabbit, Sigma-Aldrich # PRS3959 | IF (1:100) |
Anti-CD9 | Monoclonal mouse, IgG1, Invitrogen #10626D | WB (1:500) |
Anti-CD81 | Monoclonal mouse, IgG1, kappa, Invitrogen #MA5-13548 | WB (1:200) |
Anti-Flotillin1 | Polyclonal goat, IgG, Invitrogen #PA5-1853 | WB (1:1000) |
Anti-Transferrin | Polyclonal rabbit, IgG, Invitrogen #PA3-913 | WB (1:1000) |
Anti-E-Cadherin | Monoclonal rat, IgG1, kappa, eBioscience #14–3249-82 | WB (1:500) |
Anti-Syntenin | Polyclonal goat, IgG, Invitrogen #PA5-18595 | WB (1:250) |
Anti-Calnexin | Polyclonal goat, IgG, Invitrogen #PA5-19169 | WB (1:1500) |
Anti-COL1A1 | Polyclonal rabbit, IgG, ABclonal #A1352 | WB (1:1000) |
Anti-COL3A1 | Monoclonal rabbit, IgG, ABclonal #A0817 | WB (1:1000) |
Anti-β-tubulin | Monoclonal mouse, IgG2a, Invitrogen #MA5-16308 | WB (1:2000) |
Secondary antibodies | ||
Anti-mouse, AF 647 | Goat IgG, Invitrogen #A-21235 | IF (1:1000) |
Anti-mouse, AF 488 | Goat IgG3, Invitrogen #A24877 | IF (1:1000) |
Anti-mouse, AF 488 | Goat IgG, Invitrogen #A11001 | IF (1:1000) |
Anti-mouse, HRP | Goat IgG, Invitrogen #31430 | WB (1:2000) |
Anti-rabbit, HRP | Goat IgG, Invitrogen #31460 | WB (1:2000–6000) |
Anti-goat, HRP | Rabbit IgG, Invitrogen #R-21459 | WB (1:2000) |
Anti-rat, HRP | Donkey IgG, Invitrogen #A18739 | WB (1:2000) |