Table 2 Primer sequences used for qPCR
Gene name | Gene ID | Forward (5′ – 3′) | Reverse (5′ – 3′) |
|---|---|---|---|
Human genes | |||
OCT4 (Octamer-binding transcription factor 4) | 5460 | CCTTCGCAAGCCCTCATTTC | TAGCCAGGTCCGAGGATCAA |
SOX2 (SRY-box transcription factor 2) | 6657 | GGGAAAGTAGTTTGCTGCCTC | CAGGCGAAGAATAATTTGGGGG |
NANOG (Nanog homeobox) | 79923 | ACCTCAGCTACAAACAGGTGAAG | TTCTGCGTCACACCATTGCT |
B2M (beta2-microglobulin) | 567 | AATGCGGCATCTTCAAACCT | TGACTTTGTCACAGCCCAAGATA |
ACTA2 (Actin alpha 2, smooth muscle) | 59 | CTGTTCCAGCCATCCTTCAT | CCGTGATCTCCTTCTGCATT |
CCN2 (Cellular communication network factor 2; connective tissue growth factor) | 1490 | CCCCAGACACTGGTTTGAAGA | CCTCCCACTGCTCCTAAAGC |
COL1A1 (Collagen type I alpha 1 chain) | 1277 | CTTTGCATTCATCTCTCAAACTTAGTTTT | CCCCGCATGGGTCTTCA |
COL3A1 (Collagen type III alpha 1 chain) | 1281 | CTGGTGGTAAAGGCGAAATG | CCAGGAGCACCATTAGCAC |
FN1 (Fibronectin 1) | 2335 | TGTGGTTGCCTTGCACGAT | GCTTGTGGGTGTGACCTGAGT |
TAGLN (Taglin; transgelin) | 6876 | CGTGGAGATCCCAACTGGTT | AAGGCCAATGACATGCTTTCC |
TNC (Tenascin C) | 3371 | GGTCCACACCTGGGCATTT | TTGCTGAATCAAACAACAAAACAGA |
COL1A2 (Collagen type I alpha 2 chain) | 1278 | TGCTGCTGGTCAACCTGGTGC | ACTTCCAGCAGGACCGGGGG |
COL4A1 (Collagen type IV alpha 1 chain) | 1282 | CTAATCACAAACTGAATGACTTGACTTCA | AAATGGCCCGAATGTGCTTA |
TGFB1 (Transforming growth factor beta 1) | 7040 | AGGGCTACCATGCCAACTTCT | CCGGGTTATGCTGGTTGTACA |
SNAI1 (Snail family transcriptional repressor 1) | 6615 | GCTGCAGGACTCTAATCCAGA | ATCTCCGGAGGTGGGATG |
SNAI2 (Snail family transcriptional repressor 2) | 6591 | TGGTTGCTTCAAGGACACAT | GTTGCAGTGAGGGCAAGAA |
PFN1 (Profilin 1) | 5216 | ACCGCCTTCTGGTAATCTTGAG | TCCAGCATCCAGCAGACAAG |
PXN (Paxilin) | 5829 | CCCTGACGAAAGAGAAGCCTAAG | AGATGCGTGTCTGCTGTTGG |
TLN1 (Talin 1) | 7094 | CCCTGATGTGCGGCTTCG | TGTCCTGTCAACTGCTGCTTC |
SMAD2 (SMAD family member 2) | 4087 | CGTCCATCTTGCCATTCACG | CTCAAGCTCATCTAATCGTCCTG |
TGFBR2 (Transforming growth factor beta receptor 2) | 7048 | GACATCAATCTGAAGCATGAGAACA | GGCGGTGATCAGCCAGTATT |
BMPR2 (Bone morphogenetic protein receptor 2) | 659 | CTCAGTCCACCTCATTCATTTAACCG | ACAGAGACTGATGCCAAAGCAAT |
ROCK2 (Rho-associated coiled-coil containing protein kinase 2) | 9475 | CAACTGTGAGGCTTGTATGAAG | TGCAAGGTGCTATAATCTCCTC |
PFN2 (Profilin 2) | 5217 | GAGACTCTGGGTTCTAGCTGC | ACACCTTTCCCCACCAACAG |
CFL2 (Cofilin 2) | 1073 | GACTCCTTCGCTGTATCGTCT | TCTCTTTTTGATCTCCTCTTGTGT |
PTEN (Phosphatase and tensin homolog) | 5728 | CTCAGCCGTTACCTGTGTGT | AGGTTTCCTCTGGTCCTGGT |
CD44 (CD44 molecule) | 960 | CAGCTCATACCAGCCATCCAA | GACTGGAGTCCATATCCATCCTT |
CCND1 (Cyclin D1) | 595 | ATGCCAACCTCCTCAACGAC | TCTGTTCCTCGCAGACCTCC |
CCND2 (Cyclin D2) | 894 | GAAGCTGTCTCTGATCCGCA | TGCTCCCACACTTCCAGTTG |
18S rRNA (18S ribosomal RNA) | 106631781 | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | 2597 | GAAGGTCGGAGTCAACGGAT | AGTTGAGGTCAATGAAGGGGTC |
Mouse genes | |||
Acta2 (Actin alpha 2, smooth muscle) | 11475 | CAGGTGATCACCATTGGAAACGAACG | GACAGGACGTTGTTAGCATAGAGATCC |
Col1a1 (Collagen type I alpha 1 chain) | 12842 | GGAGAGAGCATGACCGATG | AAGTTCCGGTGTGACTCGTG |
Col3a1 (Collagen type III alpha 1 chain) | 12825 | TGACTGTCCCACGTAAGCAC | GAGGGCCATAGCTGAACTGA |
Ctgf (Connective tissue growth factor) | 14219 | AGACCTGTGCCTGCCATTAC | ACGCCATGTCTCCGTACATC |
Il6 (Interleukin 6) | 16193 | AGTCCTTCCTACCCCAATTTCC | TGGTCTTGGTCCTTAGCCAC |
Tnfα (Tumor necrosis factor alpha) | 21926 | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC |
Eef2 (Eukaryotic translation elongation factor 2) | 13629 | ACAATCAAATCCACCGCCATC | AGCCATCCTTGCTCTGCTTA |