Fig 4

psyAShM sites exert epiallelic effects on chromatin states and TF binding affinities. A Enrichment of 807 psyAShM sites in chromatin states. p<0.05 from Fisher’s exact tests are shown with red points (log2 (OR) and 95% confidence intervals). B Number of psyAShM sites in the DNase I hypersensitive site (DHS) and histone marks overlapping AShM loci throughout different classes of genomic elements. The P value from a binomial test is shown. C Effects of AShM on absolute TF binding affinity based on position weight matrix scores (PWMs) at predicted TF binding sites. The negative correlation between absolute TF binding affinity differences and the alternative allele hydroxymethylation level at predicted TF binding sites is shown in red, the positive correlation between absolute TF binding affinity differences and the alterative allele hydroxymethylation level at predicted TF binding sites is shown in orange, and data showing no correlation are shown in gray. D Correlation between absolute POU3F2 binding affinity differences and alternative allele hydroxymethylation level at five predicted POU3F2 binding AShM sites