Criteria | RNA-seq | EV validation | Plasma validation | Leukocyte validation | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
NP (n=7) | Preeclampsia (n=7) | P-value | NP (n=32) | Preeclampsia (n=28) | P-value | NP (n=35) | Preeclampsia (n=35) | P-value | NP (n=215) | Preeclampsia (n=38) | P-value | |
Age (years) | 29 ± 3 | 29 ± 4 | 0.653 | 32 ± 4 | 30 ± 3 | 0.037 | 32 ± 4 | 30 ± 4 | 0.019 | 32 ± 4 | 30 ± 4 | 0.001 |
Gestational age at delivery (week) | 40 ± 1 | 39 ± 2 | 0.228 | 40 ± 1 | 39 ± 3 | 0.002 | 40 ± 1 | 39 ± 3 | 0.001 | 40 ± 1 | 39 ± 3 | <0.001 |
Multiparous n (%) | 3 (43) | 2 (29) | 0.577 | 15 (47) | 8 (29) | 0.187 | 16 (46) | 10 (29) | 0.138 | 110 (47) | 11 (29) | 0.035 |
Uterine artery PI | ||||||||||||
22–24 weeks | 0.74 (0.52, 1.06) | 1.05 (0.80, 1.92) | 0.151 | 0.79 (0.72, 0.94) | 0.99 (0.79, 1.16) | 0.045 | 0.81 (0.70, 0.97) | 1.05 (0.79, 1.17) | 0.006 | 0.83 (0.72, 0.99) | 1.01 (0.79, 1.17) | 0.007 |
36–38 weeks | 0.81 (0.68, 1.24) | 0.87 (0.73, 1.03) | 1.000 | 0.72 (0.60, 0.86) | 0.84 (0.68, 1.09) | 0.060 | 0.72 (0.59, 0.85) | 0.84 (0.69, 1.08) | 0.021 | 0.72 (0.60, 0.84) | 0.84 (0.69, 1.08) | 0.007 |
BMI (kg/m2) | ||||||||||||
14–16 weeks | 23.2 (21.2, 23.6) | 23.1 (20.4, 26.6) | 0.668 | 23.9 (21.2, 26.8) | 28.1 (24.2, 30.6) | 0.002 | 23.6 (21.2, 26.4) | 27.5 (22.5, 29.8) | 0.007 | 23.5 (21.3, 25.4) | 27.4 (23.1, 29.9) | <0.001 |
22–24 weeks | 23.8 (22.4, 25.1) | 23.8 (21.7, 26.6) | 0.886 | 24.9 (22.4, 27.7) | 29.5 (25.4, 32.7) | 0.002 | 24.8 (22.4, 27.6) | 28.1 (23.2, 31.9) | 0.011 | 24.8 (22.5, 26.8) | 29.0 (23.8, 32.1) | <0.001 |
30–32 weeks | 25.0 (23.4, 25.8) | 25.1 (23.4, 28.3) | 0.886 | 27.2 (23.9, 28.8) | 31.7 (28.5, 33.4) | <0.001 | 26.4 (23.8, 28.8) | 31.3 (25.6, 33.3) | 0.004 | 26.1 (23.7, 28.3) | 31.2 (26.0, 33.3) | <0.001 |
36–38 weeks | 26.1 (24.0, 26.7) | 27.6 (24.1, 29.7) | 0.199 | 27.8 (25.2, 30.0) | 33.7 (28.2, 35.0) | 0.001 | 27.4 (25.1, 30.0) | 31.8 (27.1, 34.8) | 0.006 | 27.2 (24.8, 29.5) | 32.2 (27.3, 34.8) | <0.001 |
Systolic BP (mmHg) | ||||||||||||
14.16 weeks | 110 (110, 120) | 110 (109, 120) | 0.836 | 110 (105, 116) | 115 (110, 120) | 0.020 | 110 (105, 115) | 115 (110, 120) | 0.013 | 110 (100, 119) | 115 (110, 120) | 0.002 |
22–24 weeks | 110 (110, 120) | 110 (104, 121) | 0.836 | 110 (103, 119) | 120 (104, 120) | 0.136 | 110 (102, 115) | 118 (105, 120) | 0.119 | 110 (100, 115) | 115 (104, 120) | 0.046 |
30–32 weeks | 120 (105, 125) | 110 (105, 120) | 0.620 | 110 (106, 120) | 120 (110, 130) | 0.064 | 110 (105, 120) | 120 (110, 125) | 0.102 | 110 (105, 120) | 120 (110, 126) | 0.002 |
36–38 weeks | 118 (111, 121) | 125 (120, 150) | 0.101 | 115 (100, 120) | 130 (118, 138) | <0.001 | 115 (100, 120) | 130 (120, 139) | <0.001 | 110 (105, 120) | 130 (120, 138) | <0.001 |
Diastolic BP (mmHg) | ||||||||||||
14.16 weeks | 65 (60, 75) | 70 (69, 73) | 0.366 | 68 (60, 70) | 70 (65, 80) | 0.062 | 65 (60, 70) | 70 (65, 79) | 0.016 | 70 (60, 70) | 70 (65, 80) | 0.004 |
22–24 weeks | 70 (60, 70) | 70 (60, 80) | 0.836 | 65 (60, 70) | 70 (60, 80) | 0.035 | 75 (60, 70) | 70 (60, 80) | 0.020 | 65 (60, 70) | 70 (60, 80) | 0.001 |
30–32 weeks | 70 (60, 80) | 70 (65, 80) | 0.902 | 70 (60, 75) | 75 (70, 84) | 0.011 | 70 (60, 75) | 70 (65, 80) | 0.010 | 70 (60, 70) | 73 (70, 80) | <0.001 |
36–38 weeks | 73 (68, 80) | 80 (70, 95) | 0.181 | 70 (65, 80) | 90 (78, 93) | <0.001 | 70 (65, 80) | 85 (76, 94) | <0.001 | 70 (69, 80) | 85 (78, 93) | <0.001 |
MAP (mmHg) | ||||||||||||
14–16 weeks | 80 (77, 90) | 83 (82, 88) | 0.471 | 81 (76, 85) | 86 (80, 90) | 0.018 | 80 (75, 85) | 84 (80, 90) | 0.005 | 80 (75, 85) | 85 (80, 90) | <0.001 |
22–24 weeks | 83 (77, 87) | 84 (75, 93) | 0.999 | 80 (77, 86) | 85 (80, 93) | 0.026 | 80 (77, 85) | 85 (79, 93) | 0.016 | 80 (73, 85) | 83 (79, 93) | 0.001 |
30–32 weeks | 83 (77, 95) | 83 (77, 93) | 0.949 | 83 (77, 90) | 89 (82, 99) | 0.018 | 83 (77, 90) | 87 (82, 95) | 0.019 | 82 (77, 87) | 87 (83, 95) | <0.001 |
36–38 weeks | 88 (82, 94) | 95 (87, 113) | 0.132 | 84 (80, 92) | 103 (90,108) | <0.001 | 84 (79, 92) | 100 (90, 108) | <0.001 | 85 (80, 93) | 100 (90, 108) | <0.001 |