Fig. 2

DREEP captures single-cell variability in drug response. A Heatmap depicting both cell-line clusters (rows) and cancer-specific drug clusters (columns). Cell lines are colour-coded according to the cancer type while drugs are colour-coded according to the dataset to which they belong. Both rows and columns of the heatmap were grouped in four clusters named C1–C4 for cell line (rows) and D1–D4 for drugs (columns). B Bar-plot depicting enriched MoAs in each cancer-specific drug cluster. Only the top ten significant MoAs (FDR < 10%) for each cluster are shown. C UMAP plot of 7805 cells of 24 cell lines either untreated or exposed to nutlin treatment. D The Spearman correlation coefficient (SCC) between observed and predicted sensitivity scores for the XX untreated (DMSO) cell lines in C. In the upper plot, the SCC is computed using cells sequenced after 6 h, while in the bottom plot, SCC is computed using cells collected after 24 h. E Same as in D but for nutlin-treated cells