Fig. 4

Maternal sevoflurane exposure did not affect the postnatal distribution of cortical interneurons. A Experimental protocols. B Sample images showing the co-expression of YFP+ neurons with PV and SST. PV, parvalbumin. SST, somatostatin. Scale bar: 10 μm. C Representative images showing the laminar distribution of YFP+ cells at P7, , and P30; PV+ and SST+ cells at P30. The crops were organized into 6 layers which could be demarcated by DAPI. Scale bar: 200 μm. D Quantity and percentages of YFP+ cells located in different layers at P7. E Quantity and percentages of YFP+ cells located in different layers at P15. F Quantity and percentages of YFP+ cells located in different layers at P30. G-H Quantity and percentages of PV+ and SST+ cells located in different layers at P30. Ctr: 18 slices from 3 mice; Sevo: 18 slices from 3 mice. The data were represented as mean ± SEM. Two-tailed Student’s t-test was used for quantity comparisons. Two-way ANOVA was used for analyzing the laminar distribution