Fig. 5

Maternal sevoflurane exposure alters local neural microcircuitry in the cortex of adolescent offspring. A Representative traces showing voltage responses of fast-spiking interneurons in Ctr and Sevo groups to step current injections. Red traces indicate the first evoked AP. Ctr: 9 cells from 3 mice; Sevo: 6 cells from 3 mice. B–F Comparisons about the AP threshold (B), AP amplitude (C), AP half-width (D), input resistance (E), and resting membrane potential (F) of fast-spiking interneurons. Ctr: 9 neurons from 3 mice; Sevo: 6 neurons from 3 mice. G Spike number change of fast-spiking interneurons with different current injections. Ctr: 6 neurons from 3 mice; Sevo: 6 neurons from 3 mice. H–K The neurons in cortex of P30 mice were co-immunostained with NeuN/GAT1 or NeuN/Gephyrin in (H); NeuN/Glut1 or NeuN/PSD95 in (I); PV/GAT1 or PV/Gephyrin in (J); PV/Glut1 or PV/PSD95 in (K); The white dotted circle represented the region of interest for boutons count. Scale bars: 5 μm. L–-O The differences of excitatory/inhibitory synaptic boutons puncta around the cell soma of NeuN+YFP− (excitatory) (L, M) or PV+ (inhibitory) (N, O) neurons between Ctr and Sevo group were analyzed. 7–19 cells from 3 mice per group in each condition. P Coronal slices of cortex were co-immunolabeled with Glut1/GAT. Scale bar: 10 μm. Q Synaptic puncta were calculated, and the ratio of the integrated density of excitatory and inhibitory puncta in the cortex was shown. 5–7 slices from 3 mice per group. The data are represented as mean ± SEM. Two-tailed Student’s t-test was performed for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.00, n.s. no significance