Fig. 6

The CXCL12/CXCR4 signaling pathway is involved in sevoflurane-induced defects in interneuron migration. A Q-PCR analysis. Ctr: n=3; Sevo: n=3. B Representative ISH images. Scale bar: 200 μm. C Experimental protocols for overexpressing CXCL12 in the embryonic cortex by conducting IUE at E13.5. D Representative images of sections from E14.5 embryonic brains in the Ctr group, Sevo group, CXCL12+RFP Sevo group (offspring hemispheres electroporated with CXCL12 and RFP at E13.5 and exposed to sevoflurane at E14.5), and RFP Sevo group (offspring hemispheres electroporated with RFP without CXCL12 at E13.5 and exposed to sevoflurane at E14.5). Scale bar: 50 μm. E Radial distribution of YFP+ cells. F Quantification of leading process orientation into four quadrants. M, medial; V, ventral; L, lateral; D, dorsal. Ctr: 129 cells from 3 mice; Sevo: 118 cells from 3 mice; CXCL12+RFP Sevo: 154 cells from 3 mice; RFP Sevo: 134 cells from 3 mice. The data are represented as mean ± SEM. Two-tailed Student’s t-test (A) and one-way ANOVA (E, F) were performed for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001