Fig. 7
The overexpression of CXCL12 rescues the increased epilepsy susceptibility and ameliorates abnormal behaviors in adolescent offspring. A Experimental design. B Quantification of total body weight in the four groups. C Representative images showing the expression of RFP in the cortex which indicated the electroporation. Scale bar: 250 μm. D Comparisons about the latency to induce minimal seizures. E Comparisons about the cumulative doses required to induce the 1st tonic-clonic seizures. Ctr+RFP vs Sevo+RFP: p=0.0697. F Comparisons about the latency required to induce the 1st tonic-clonic seizures. Ctr+RFP vs Sevo+RFP: p=0.0413. G Comparisons about the intervals between minimal and tonic-clonic seizures. Ctr+RFP vs Sevo+RFP: p=0.0430. H The representative traces in the OFT within 10 min and the representative traces in the EPM in 5 min. I-L Comparisons about the total distance traveled (I), the central distance traveled (J), the number of central visits (K), and the percentage of central time (L). Ctr+RFP vs Sevo+RFP: p=0.0153, 0.0118, 0.0413, and 0.0463, respectively. M–O Comparisons about the total number of arm entries (M), the ratio of open/total arm entries (N), and the ratio of the duration in open arm/total time (O). No significance between the groups. P The immobile time in the TST. Ctr+RFP vs Sevo+RFP: p=0.0316. Ctr + RFP: n=13, Ctr + OE CXCL12: n=12, Sevo + RFP: n=12, Sevo + OE CXCL12: n=12. The data are represented as mean ± SEM. One-way ANOVA was performed for statistical analysis. *p < 0.05