Fig. 2

Myeloid subpopulations in ARVC human hearts. A UMAP embedding of 18 myeloid subpopulations. B Dot plot showing the top five marker genes of each subcluster. Dot color and size correspond to the expression of each gene and the proportion of cells expressing each gene, respectively. C The ratio of each subcluster in the different phases and topographic regions. D, E Trajectory analysis of selected clusters. F Multiple labeling staining for CCL3⁺ CD68⁺ macrophages; scale bar indicates 100 μm. Each spot represents one sample. Data are mean ± SD. Mann–Whitney U test was performed to compare the cellular ratio of Mye2 between ARVC and NC. UMAP, uniform manifold approximation and projection; ARVC, arrhythmogenic right ventricular cardiomyopathy; NC, normal control; AC_LV, left ventricle of ARVC; AC_RV, right ventricle of ARVC; NC_LV, left ventricle of NC; NC_RV, right ventricle of NC; AC_PBMC, PBMC of ARVC; PBMC, peripheral blood mononuclear cell; MP, macrophage; MO, monocyte; DC, dendritic cell; DEGs, differentially expressed genes. Gene names mentioned in the main text are color-coded