Fig. 5

MLL1 regulates trophoblast syncytialization through TEAD4 expression. A Genomic distribution of MLL1 and H3K4me3 peaks in BeWo cells. B Genome browser view of normalized H3K4me3 and MLL1 CUT&Tag signals for known target genes. C Venn diagram showing the overlap between genes with promoters bound by H3K4me3 (n = 14,482), genes with promoters bound by MLL1 (n = 7097), and differentially expressed genes in the RNA sequencing (RNA-seq) data (n = 798). D KEGG analyses performed using the 342 intersection genes. E Heatmap of downregulated intersection genes that are related to the Hippo signaling pathway according to the RNA-seq data. F Western blots of TEAD4 in MLL1-downregulated or MLL1-upregulated BeWo cells. G Genome browser view of CUT&Tag signals and RNA-seq tracks for TEAD4 in BeWo cells. H The expression levels of MLL1 and TEAD4 mRNA correlate positively in multiple human organs according to the GTEx database. I-J Quantitative ChIP analysis of H3K4me3 at the TEAD4 promoter in FSK-treated or MLL1-knockdown BeWo cells. The values are normalized to IgG. K Quantitative ChIP analysis of MLL1 at the TEAD4 promoter in FSK-treated BeWo cells. The values are normalized to IgG. L Western blots of MLL1, TEAD4, and HCG in BeWo cells exposed to si-MLL1, with or without TEAD4 overexpression. Data are presented as the means ± SD. **P < 0.01, *P < 0.05. TEAD4, TEA domain transcription factor 4; ChIP, chromatin immunoprecipitation