Fig. 3

PREX2 inhibits radiosensitization of CRC in vivo. A–C Subcutaneous tumor formation in nude mice was established with PREX2-overexpressing or control RKO cells and treated with or without IR (n = 6/group). A The representative image of tumors derived from xenograft mice. B, C The volume and weight of tumors were monitored. Data were shown as means ± SEM. D–F Subcutaneous tumor formation in C57/B6J mice was established with PREX2- knockdown or control CMT93 cells and treated with or without IR (n = 6/group). D Representative images of each group were photographed at the end of the experiment. E, F The volume and weight of tumors were monitored. Data were shown as means ± SEM. G Subcutaneous tumors in nude mice were stained with hematoxylin and eosin (H&E, top). The representative IHC images of cleaved-caspase 3 and γH2AX expression in xenograft tumors were shown (below). Scale bar: 100 μm. *p < 0.05, **p < 0.01,***p < 0.001