Fig. 4

PREX2 modulated radiation-induced DNA damage response by regulating NHEJ repair. A Representative fluorescence images of γH2AX staining. Scale bar: 10 μm. B Quantification of γ-H2AX immunostaining in PREX2 overexpressed RKO cells and PREX2 knocked down SW480 cells with or without 4 Gy radiation. C, D The comet assay of stable overexpression and interference cell lines after IR treatment (scale bar: 50 μm, C) and the statistics result of quantification of tail moment (D). E Western blot detection of the expression levels of DNA-PKc, p-DNA-PKc, p-ATM, ATM, p-ATR, ATR, p-CHK1, and CHK1 in stable overexpression and interference cell lines with or without 4 Gy radiation. F Bioinformatics analysis of the correlation between PREX2 and genes related to NHEJ pathway (XRCC6, XRCC5, LIG4, PARP1, TOPBP1 and PRKDC) in TCGA-CRC. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001