Fig. 6

PREX2 inhibited radiation-induced immunogenic cell death and CD8⁺ T cell infiltration. A Immunofluorescence assay detection of the calreticulin (CRT) expression in PREX2 overexpressed RKO cells at 24 h after 4 Gy radiation. Scale bar: 20 μm. B Immunofluorescence assay detection of the CRT expression in PREX2 knocked down SW480 cells at 24 h after 4 Gy radiation. Scale bar: 10 μm. C Flow cytometry analysis confirmed that overexpressing PREX2 reduced CRT protein levels. D Detection of adenosine triphosphate (ATP) secretion by luciferin-based ATP assay kit. E Detection of high mobility group protein B1 (HMGB1) release by ELISA kit. F The representative images of HMGB1 immunofluorescence staining of xenograft tumors were shown (left). Scale bar: 10 μm. Quantification of HMGB1 means fluorescence intensity was analyzed (right). G The correlations between PREX2 expression and the immune score of CD8⁺ T cells were analyzed by the EPIC algorithm in TCGA-CRC. H Representative HE images and IHC images of CD3 and CD8 staining (left) and statistical analysis (right) of CMT93 tumors. Scale bar: 100 μm. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001