Open Access

Erratum to: CD14 hi CD16+ monocytes phagocytose antibody-opsonised Plasmodium falciparum infected erythrocytes more efficiently than other monocyte subsets, and require CD16 and complement to do so

  • Jingling Zhou1,
  • Gaoqian Feng1,
  • James Beeson1, 2, 6,
  • P. Mark Hogarth1,
  • Stephen J. Rogerson2,
  • Yan Yan3 and
  • Anthony Jaworowski1, 4, 5Email author
BMC Medicine201513:290

https://doi.org/10.1186/s12916-015-0530-1

Published: 30 November 2015

The original article was published in BMC Medicine 2015 13:154

Erratum

It has come to the publisher's attention that the original version of this article [1] contained an error in Fig. 1. Panel 1e was an inadvertent duplication of panel 1b. The correct Fig. 1 has been published in its entirety below.
Fig. 1

CD14 hi CD16+ intermediate monocytes phagocytose IE more efficiently than other monocytes. a Whole blood was incubated with EtBr-labelled CS2-IE for 30 min then uningested RBC removed by hypotonic lysis and washing. Cells were stained with anti-CD14 and CD16, monocytes gated using forward and side scatter then subsets defined as classical (C: CD14 hi CD16-), intermediate (IM: CD14 hi CD16+) and non-classical (NC: CD14 lo CD16+) as shown. Histograms show EtBr staining of the three subsets incubated at 37 °C (red histograms) or 4 °C (blue histograms) with unopsonised (IE, top) or opsonised (IgG-IE, bottom) IE. b Phagocytosis using blood from eight separate donors. Whole blood was incubated as in awith unopsonised CS2-IE (left hand panels; IE) or CS2-IE opsonised with rabbit anti-human RBC antibody (right hand panels; IgG-IE) as indicated. c Phagocytosis by monocyte subsets of IE opsonised with rabbit anti human RBC was measured using PBMC prepared from four separate donors (left hand panels). Phagocytosis of IE opsonised with pooled human immune serum was measured using PBMC prepared from six separate donors (right hand panels). d Phagocytosis of unopsonised CS2-IE (left hand panels; IE) and CS2-IE opsonised with pooled human immune serum (right hand panels; IgG-IE) was measured in a whole blood assay as in a using blood from nine separate donors. e Phagocytosis using blood from six separate donors. Whole blood was incubated as in a with unopsonised E8B-IE (left hand panels; IE) or E8B-IE opsonised with rabbit anti-human RBC antibody (right hand panels; IgG-IE) as indicated. Background phagocytosis measured at 4 °C was subtracted from all data points. The percent phagocytosis by intermediate (IM) monocytes was compared using pairwise comparisons in each case (b-e) with either that by classical (C) monocytes or non-classical (NC) monocytes, as indicated. Differences between groups were assessed using Wilcoxon matched pairs signed rank test: * p < .05, ** p < 0.01. EtBrethidium bromide, IE infected erythrocytes, PBMC peripheral blood monocytes; RBC red blood cells

Notes

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Centre for Biomedical Research, Burnet Institute
(2)
Department of Medicine, University of Melbourne
(3)
Department of Chemical and Biomolecular Engineering, University of Melbourne
(4)
Department of Infectious Diseases, Monash University
(5)
Department of Immunology, Monash University
(6)
Department of Microbiology, Monash University

Reference

  1. Zhou J, Feng G, Beeson J, Hogarth PM, Rogerson SJ, Yan Y, et al. BMC Med. 2015;13:154.Google Scholar

Copyright

© Zhou et al. 2015

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