Fig. 2

Analysis of BCL-2 family proteins in the chemo-resistant 1889c reveals an MCL-1-dependent apoptotic priming. A Dose-response curve of 1889c and B HCC15 for cisplatin and etoposide (0.1–10μM) and sunitinib and sorafenib (0.1–10μM). C Western blot analysis of TP53, MCL-1, BCL-2, BCL-xL, BAX, BAK, and NOXA in 1889c and HCC15. D BH3 profiling shows priming for MCL-1 in 1889c and BCL-2/BCL-xL in HCC15. E Dose-response curve of 1889c after a 24-h single treatment with AZD5991 and A-1155463 (0.01–1μM) and combined treatment with AZD5991 and A-1155463 (0.05μM). F Cell viability and G PI staining of 1889c after a 24-h single treatment with AZD5991 (0.5μM) and A-1155463 (0.05μM) and the combination of AZD5991 and A-1155463. H Western blot analysis of PARP cleavage and caspase activation of 1889c after a 6-h single treatment with AZD5991 (0.5μM) and A-1155463 (0.05μM) and the combination of AZD5991 and A-1155463. I Dose-response curve of 1889c after a 24-h single treatment with AZD5991, ABT-263, and AZD5991 (0.01–1μM) in combination with ABT-263 (0.5μM). J Cell viability and K PI staining of 1889c after a 24-h single treatment with AZD5991 (0.5μM) and ABT-263 (0.5μM) and the combination of AZD5991 and ABT-263. L Analysis of PARP cleavage and caspase activation of 1889c after a 6-h single treatment with AZD5991 (0.5μM) and ABT-263 (0.5μM) and the combination of AZD5991 and ABT-263 by western blot