Fig. 5
BH3 profiling of primary patient tissue samples predicts apoptotic priming and therapy response to BH3 mimetics. A Correlation between IHC MCL-1 staining intensity and relative gene copy number (CN) of MCL-1 (n=32, 5 A, 13 AB, 1 B1, 5 B2, 4 B3, and 4 TC) (p=0.0460*). B Significantly better overall survival for patients with low copy numbers of MCL-1 (MCL-1-CNlow) (p=0.0222*). C, D BH3-profiling of a healthy thymus (NT), two type B3 (TH-1 and 2), a type B2 (TH-3), and a type AB (TH-4) TH revealed marked priming for MCL-1/BCL-xL in the type B3 TH. E Western blot analysis of MCL-1, BCL-2, and BCL-xL expression in the same tissue samples. F, G BH3 profiling and H western blot analysis of a healthy lung (NL) and three SCCL samples