Change in habitual intakes of flavonoid-rich foods and mortality in US males and females
BMC Medicine volume 21, Article number: 181 (2023)
Higher baseline intakes of flavonoid-rich foods and beverages are associated with a lower risk of chronic disease and mortality in observational studies. However, associations between changes in intakes and mortality remain unclear. We aimed to evaluate associations between 8-year changes in intakes of (1) individual flavonoid-rich foods and (2) a composite measure (termed the ‘flavodiet’) of foods and beverages that are known to be main contributors to flavonoid intake and subsequent total and cause-specific mortality.
We evaluated associations between 8-year changes in intakes of (1) individual flavonoid-rich foods and (2) a novel ‘flavodiet’ score and total and cause-specific mortality. We included 55,786 females from the Nurses’ Health Study (NHS) and 29,800 males from the Health Professionals Follow-up Study (HPFS), without chronic disease at baseline in our analyses. Using multivariable-adjusted Cox proportional hazard models, we examined associations of 8-year changes in intakes of (1) flavonoid-rich foods and (2) the flavodiet score with subsequent 2-year lagged 6-year risk of mortality adjusting for baseline intakes. Data were pooled using fixed-effects meta-analyses.
We documented 15,293 deaths in the NHS and 8988 deaths in HPFS between 1986 and 2018. For blueberries, red wine and peppers, a 5%, 4% and 9% lower risk of mortality, respectively, was seen for each 3.5 servings/week increase in intakes while for tea, a 3% lower risk was seen for each 7 servings/week increase [Pooled HR (95% CI) for blueberries; 0.95 (0.91, 0.99); red wine: 0.96 (0.93, 0.99); peppers: 0.91 (0.88, 0.95); and tea: 0.97 (0.95, 0.98)]. Conversely, a 3.5 servings/week increase in intakes of onions and grapefruit plus grapefruit juice was associated with a 5% and 6% higher risk of total mortality, respectively. An increase of 3 servings per day in the flavodiet score was associated with an 8% lower risk of total mortality [Pooled HR: 0.92 (0.89, 0.96)], and a 13% lower risk of neurological mortality [Pooled HR: 0.87 (0.79, 0.97)], after multivariable adjustments.
Encouraging an increased intake of specific flavonoid-rich foods and beverages, namely tea, blueberries, red wine, and peppers, even in middle age, may lower early mortality risk.
Findings from prospective cohort studies, supported by recent randomised controlled trials, provide growing evidence that higher intakes of plant-based foods and beverages, rich in bioactive compounds such as flavonoids, may offer distinct benefits in relation to health and the prevention of premature death [1,2,3,4]. This is attributed to the role of flavonoids in protecting against many chronic diseases, as demonstrated by converging evidence from in vitro, clinical and epidemiological studies [5, 6]. Flavonoids are the most abundant and most researched class of polyphenols, a major group of phytochemicals which demonstrate important biological effects . Although nearly all plant-based foods contain flavonoids, some contain uniquely high concentrations, namely tea, apples, berries, citrus fruits, dark chocolate and red wine.
Many studies on habitual food intake and health are derived from a single, baseline dietary intake measure; repeated measures of intake are preferable as benefits are likely cumulative for most chronic disease and more up-to-date consumption level data captures within-person variability over the adult lifetime . A recent approach is to report longitudinal analyses of changes in consumption of foods and relate these to health outcomes. Examining associations for change in intake is valuable as eating behaviours change over time  and it is important to know if such change (either an increase or a decrease) can influence health outcomes. These data would provide public health officials an evidence base to give consumers clear advice regarding whether changing from being a low consumer to a high consumer, or vice versa, could have a real impact on health, even in mid-life. Moreover, as the data are derived within individuals, this approach is less prone to bias and confounding, and more closely resemble findings observed in randomised controlled trials .
The average intake of total flavonoids in the US (∼250 to ∼400 mg/day) is lower than the UK and Australia, and in line with intakes in Europe and China , with major dietary sources being tea, citrus fruit juices, berries, citrus fruit, wine, and apples . While previous studies have demonstrated that higher intakes of specific flavonoid subclasses are associated with a lower risk of mortality [1, 4, 12], to our knowledge no studies have examined associations between changes in intakes of flavonoid-rich foods and risk of all-cause and cause-specific mortality. As foods are consumed as a whole, exploring associations for flavonoid-rich foods, as opposed to total flavonoids or flavonoid subclasses, yields results with a clearer interpretation and public health relevance. Flavonoids are found in a wide variety of plant-based foods and beverages, yet across the globe the major dietary sources of flavonoids tend to be very similar [13,14,15,16]; as such, deriving a composite score of flavonoid-rich foods is a novel and valuable method to better understanding the role of dietary flavonoids in disease prevention.
We therefore aimed to evaluate associations between 8-year changes in intakes of (1) individual flavonoid-rich foods and (2) a composite measure (termed the ‘flavodiet’) of foods and beverages that are known to be main contributors to flavonoid intake and subsequent total and cause-specific mortality among participants in the Nurses’ Health Study (NHS) and the Health Professionals Follow-up Study (HPFS). We hypothesised, on the basis of previous findings, that increases in intakes of some flavonoid-rich foods and beverages (namely, blueberry, apple, tea, and red wine) would be associated with a lower risk of all-cause mortality and that increases in the flavodiet score would be associated with a lower risk of cardiovascular disease-related, respiratory-related, and neurological disease-related mortality.
Data from two large prospective cohorts of US males and females were used for this study: the NHS began in 1976 and enrolled 121,701 female nurses aged 30–55 years  while the HPFS began in 1986 and enrolled 51,529 male health professionals aged 40–75 years . In both cohorts, participants completed questionnaires about their lifestyle, and medical history at baseline and were invited to repeat these questionnaires every subsequent 2 years to collect and update information on lifestyle, and occurrence of new-onset diseases; follow-up rates for mortality exceed 98% for both cohorts.
The baseline for all analyses in the present study was set to be 1994, 8 years after detailed information on diet, physical activity, and other lifestyle factors had been collected for both cohorts, and the end of follow-up was 2018. We excluded participants who had prevalent cardiovascular disease, diabetes, cancer, or severe chronic neurological disease 4 years after baseline to reduce the likelihood of observed associations being due to reverse causality, participants with extreme energy intakes (< 800 or > 4200 kcal/d for men and < 600 or > 3500 kcal/d for women) which may reflect incorrect completion of the FFQ, and participants with missing information on change in flavonoid intakes and change in total fruit intakes. The final analysis included 55,786 females and 29,800 men (Additional file 1: Fig. S1). The last observation was carried forward for missing values of continuous variables (with the exception of diet) and indicators were used for missing values of categorical variables. Missing values were carried forward only once for diet, after which the follow-up was censored.
The study protocol was approved by the institutional review boards of the Brigham and Women’s Hospital and Harvard T.H. Chan School of Public Health, and those of participating registries as required.
Participants of the two cohorts completed validated semi-quantitative food frequency questionnaires (FFQs) in 1986 and were invited to repeat these questionnaires every 4 years thereafter. Participants were asked how often, on average, they consumed a standard portion of each food and beverage in the past year with frequency response categories ranging from never or less than once a month, to six or more times per day. Exposures of interest were changes in intakes (servings/day) of foods and beverages that are known to be main contributors to flavonoid intake [namely, blueberry (½ cup), apple (1 fruit), orange (1 fruit) plus orange juice (1 small glass), grapefruit (½ fruit) plus grapefruit juice (1 small glass), strawberry (½ cup), tea (8 oz. cup), red wine (5 oz. glass), onion (1 slice raw or ½ cup cooked), peppers (2 rings or ¼ small), and grapes (½ cup) plus raisins (1 oz. or 1 small pack)] [16, 19]. Secondly, intakes of flavonoid-rich foods/beverages that contributed > 1% to total flavonoid intakes in both the NHS and the HPFS at all waves of follow-up were summed to create a ‘flavodiet’ score [i.e., tea, apples, oranges, grapefruits, blueberries, strawberries and red wine (servings/day)]. Of note, chocolate intake was not included as an exposure of interest as FFQs prior to 2006 did not assess intakes of milk and dark chocolate separately. The reproducibility and validity of the FFQs have been described previously [20, 21]. For the NHS, the corrected correlation coefficients, for several key flavonoid-rich foods, between the food frequency questionnaire and multiple dietary records were between 0.74 and 0.80 for apples, 0.50 and 0.74 for oranges, 0.86 and 0.93 for tea, and 0.83 and 0.90 for wine . For the HPFS, these corrected correlation coefficients were between 0.44 and 0.53 for apples, 0.40 and 0.43 for oranges, 0.62 and 0.69 for tea, and 0.56 and 0.60 for red wine .
Ascertainment of mortality
Death from any cause was the primary outcome for these analyses. Deaths were identified using the state vital statistics records, the national death index, reports by families, and the postal system; we ascertained over 98% of deaths in each cohort . For all deaths, death certificates were sought and, when appropriate, permission was requested from the next of kin to review medical records. These death certificates and medical records were reviewed by a physician to determine the underlying cause of death according to the eighth and ninth revisions of the International Classification of Diseases (ICD) codes. Causes of death were grouped into five categories: cardiovascular disease-related, cancer-related, respiratory-related, neurological disease-related, and other). Due to heterogeneity in causes of death, the ‘other’ category, was not included it as an outcome of interest.
Assessment of covariates
Participants reported their demographics (age, ethnicity, weight and height), lifestyle habits (physical activity, smoking habits, aspirin and multivitamin use), family history of diseases (myocardial infarction, diabetes and cancer) and any recent physician-diagnosed diseases (myocardial infarction, diabetes, cancer, hypertension, and hypercholesterolemia) via a questionnaire every 2 years. Physical activity was quantified as energy expenditure in metabolic equivalent tasks (METs) measured in hours per week as described previously . As there was approximately 10% missing data for each question, response rates were carried over from the previous questionnaire. Dietary intakes of alcohol, total energy, meat, nuts, saturated fat, polyunsaturated fat, trans-fat, cereal fibre, and soft drink were assessed and updated from the food frequency questionnaire every 4 years.
We calculated person-years of follow-up from the date of return of the 1994 questionnaire to the date of death or the end of follow-up, whichever came first. We used time-dependent Cox proportional hazards regression to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) of total and cause-specific mortality in the subsequent 6 years, after excluding the first 2 years of follow-up time in a lagged analysis; that is, changes in consumption between 1986 and 1994 predicted mortality between 1996 and 2002; changes in consumption between 1994 and 2002 predicted mortality between 2004 and 2010, and changes in consumption between 2002 and 2010 predicted mortality between 2012 and 2018. Proportional hazards assumptions were checked with no violations found. For the individual foods, participants were divided into seven categories based on their changes in consumption: three increase categories (increase of 0.5–0.99 servings/week; increase of 1–1.99 servings/week; increase of ≥ 2 servings/week); three decrease categories (decrease of 0.5–0.99 servings/week; decrease of 1–1.99 servings/week; decrease of ≥ 2 servings/week), and one reference category (no change; + / − < 0.49 servings per week). Exposures were categorised as we did not wish to assume that associations were linear, a reference value of ‘no change’ was chosen so that different associations for an increase and a decrease in intake could be observed, and the category cut points were chosen based on the distribution of the data and ease of interpretation and translation. For the flavodiet score, participants were divided into seven categories based on their changes in consumption: three increase categories (increase of 1–3.9 servings/week; increase of 4–6.9 servings/week; increase of ≥ 7 servings/week); three decrease categories (decrease of 1–3.9 servings/week; decrease of 4–6.9 servings/week; decrease of ≥ 7 servings/week), and one reference category (increase or decrease of < 1 serving per week). Updated 8-year changes in consumption of individual flavonoid-rich foods, or the composite flavodiet score, were used as time-varying exposures. Eight years was chosen to allow enough time for people to change their diet and for that change to have an effect, as shown previously . Risk of death (HR and 95% CI) was also estimated for a change in intake of each individual food or beverage by 3.5 servings/week, representing a serving every second day, except for tea intake which was estimated for 7 servings/week, representing a serving every day. For the flavodiet score, the risk of death (HR and 95% CI) was also estimated for a change total intake of 3 servings/day. We calculated HRs and 95% CIs from the different models separately for each cohort and then pooled the results of Model 2 from both cohorts using a fixed-effects meta-analysis. As the between-study variance heterogeneity tests were not significant for most of the main exposures, a fixed-effects meta-analysis was used.
Model 1 adjusted for baseline age (calendar year) and baseline intake of the exposure variable of interest while Model 2 adjusted for baseline age, follow-up time periods, ethnicity (white v other), change in smoking status (never to never, never to current, former to former, former to current, current to former, current to current, or missing indicator), a family history of myocardial infarction, diabetes and cancer (all yes v no), multivitamin use (yes v no), aspirin use (yes v no), history of hypertension, hypercholesterolemia, and diabetes (all yes v no), baseline physical activity (quintiles), change in physical activity, baseline BMI (< 23, 23–24.9, 25–29.9, 30–34.9, > 35 kg/m2), change in BMI, baseline intakes of the exposure variable of interest, and both baseline and change in intakes of alcohol, total energy, meat, nuts, saturated fat, polyunsaturated fat, trans fat, cereal fibre, and soft drink (all servings/day).
To investigate potential effect modification of the association between change in the flavodiet score and mortality (all-cause and cause-specific), we stratified our analyses by BMI (< 30 v ≥ 30 kg/m2) and smoking status (never v ever) based on prior evidence of effect modification . Furthermore, we conducted sensitivity analyses where we censored participants at 80 years of age so that our outcome would represent premature death. All analyses used SAS version 9.2 (SAS Institute, Cary, NC) and statistical tests were two-sided and a P value < 0.05 was considered statistically significant.
We documented 15,293 deaths (including 3401 CVD deaths, 3530 cancer deaths, 1282 respiratory deaths and 2475 neurological deaths) in the NHS over 843,190 person-years of follow-up and 8988 deaths (including 2634 CVD deaths, 2345 cancer deaths, 677 respiratory deaths and 828 neurological deaths) in HPFS over 423,565 person-years of follow-up. Table 1 shows the characteristics of the participants based on an 8-year change in the flavodiet score from 1986 to 1994. From 1986 to 1994, more participants increased their intakes of flavonoid-rich foods than decreased their intakes in both cohorts (Table 1). Although body weight tended to increase over follow-up for all participants, those with the greatest increase in flavonoid-rich food intake appeared to be healthier in that they had, on average, a lower increase in body weight and a greater increase in both energy intake and physical activity than participants who decreased their flavonoid-rich food intake. Participants who maintained a similar level of flavonoid-rich food intake were more likely to be current smokers than those who increased or decreased their intakes. Mean intakes of most flavonoid-rich foods remained stable over time (1986 to 2010) except for intakes of blueberries, tea, and red wine, which tended to increase in both cohorts, and intakes of citrus fruits (orange and grapefruit) and their juices which tended to decrease (Additional file 1: Fig. S2).
Age-adjusted (Model 1) associations between changes in intakes of flavonoid-rich foods and total mortality are presented in Additional file 1: Table S1. In pooled multivariable-adjusted analyses, compared with participants whose intakes remained relatively stable, those with the greatest increase in intakes of tea, red wine and peppers had a 5%, 11%, and 6% lower risk of total mortality, respectively [pooled HR (95% CI) for tea: 0.95 (0.92, 0.98); red wine: 0.89 (0.84, 0.95); and peppers: 0.94 (0.90, 0.98); Table 2]. When modelled as a continuous variable, a 1 serving/day increase in tea intake was associated with a 3% lower all-cause mortality while 1 serving every other day (3.5 servings/week) increases in intakes of red wine, peppers and blueberries were associated with 4%, 9% and 5% lower risks of all-cause mortality in multivariable-adjusted pooled analyses, respectively. For blueberries, this appeared to be driven by a trend for a high risk of mortality in participants who decreased their intakes of blueberries. In contrast, those with the greatest increase in intakes of strawberries, onions and citrus fruits and their juices had an 8% (strawberry), 6% (onion), 8% (orange) and 6% (grapefruit) higher risk of mortality after multivariable adjustments (Table 2). When modelled as a continuous variable, a 1 serving every other day (3.5 servings/week) increase in intakes of grapefruit and onion was associated with a 6% and 5% higher risks of all-cause mortality in multivariable-adjusted pooled analyses, respectively.
When intakes of the top contributors to total flavonoid intake were combined to create a flavodiet score, a 3 servings/day increase in this combined score was associated with an 8% lower risk of all-cause mortality, after multivariable adjustments [pooled HR (95% CI): 0.92 (0.89, 0.96); Fig. 1]. Participants who had the greatest decrease in flavodiet score (≥ 7 servings/week) had an 11% higher risk of mortality [pooled HR (95% CI): 1.11 (1.05, 1.17)] than those whose score remained stable (Table 3). Although an increase in flavodiet score was not significantly associated with a reduction in risk of cardiovascular, cancer or respiratory mortality, an increase of 3 servings per day of the flavodiet score was associated with a 13% reduction in risk of neurological death (Fig. 1) and participants who had the greatest decrease in flavodiet score (≥ 7 servings/week) had a 14% higher risk of neurological mortality [pooled HR (95% CI): 1.14 (1.00, 1.31)] than those whose score remained stable (Table 3).
In sensitivity analyses, censoring participants at 80 years did not materially change the results. Furthermore, we found no evidence of effect modification by smoking status (ever v never) or BMI when examining associations between the flavodiet score and either all-cause or cause-specific mortality in each cohort (pinteraction > 0.1 for all).
In this prospective cohort study of well-characterised US females and males with repeated measures of dietary intake to allow for the calculation of an 8-year change in consumption of flavonoid-rich foods and beverages, we observed that an achievable increase in intake of several flavonoid-rich foods, namely tea, blueberries, red wine and peppers, was associated with up to an 11% reduction in all-cause mortality. Furthermore, by deriving a novel flavodiet score we showed that an increase of three servings per day of any of the main contributors to total flavonoid intake was associated with an 8% and 13% lower risk of all-cause and neurological death, respectively. These novel findings highlight that modifying eating behaviours in mid to later life can influence health outcomes and that several flavonoid-rich foods may offer distinct benefits.
To date, most observational studies aiming to understand the role of dietary flavonoids in the prevention of chronic disease and subsequent early mortality have focussed on total flavonoid intakes and intakes of major flavonoid subclasses and compounds . However, the overall health effect may depend on the other components that they are consumed alongside as part of the whole food matrix . Thus, there is also a need to evaluate flavonoids in the context of the whole food; one way in which this can be done is by examining associations between intakes of flavonoid-rich foods and health outcomes. In the present study, increased intakes of not all flavonoid-rich foods were associated with a lower risk of total mortality. Of the fruits, decreasing intakes of blueberries and increasing intakes of strawberries and citrus fruits and their juices were associated with a higher risk of total mortality. In the case of strawberries, decreasing intakes were also associated with a higher risk of mortality and for both strawberries and oranges the test for trend was not statistically significant; thus, findings are less convincing and may be due to chance, particularly given the large number of statistical tests undertaken. The differential associations for blueberries and citrus, grapefruit in particular, may be explained by other, non-beneficial, properties of the citrus fruits and juices, namely the high glycaemic load of juice  and the known interaction between grapefruit and drug metabolism . Regarding flavonoid-rich vegetables, a 3.5 servings per week increase in onion intake was associated with a 5% higher risk of mortality while the same increase in intakes of peppers was associated with a 9% lower risk of all-cause mortality. As onions and peppers are rarely eaten in isolation, their health effects are inherently difficult to disentangle from effects of the whole meal in an observational setting and the observed associations should be interpreted with caution. Associations observed in the present study are comparable to those reported previously for the NHS II cohort of younger females, where frequent consumers of blueberries, peppers, red wine and tea had a lower risk of mortality while grapefruit consumers were at a higher risk . Conversely, no association was observed between onion intakes and mortality in the NHS II. Plant-derived beverages are also an important dietary source of bioactive compounds including flavonoids. In our pooled analyses, increases in tea and red wine intakes were inversely associated with all-cause mortality risk, in line with findings from previous meta-analyses [30, 31]. Based on findings from both epidemiological and interventional studies, a small to moderate intake of red wine daily is thought to be cardioprotective, with evidence of a synergistic effect between the polyphenols and the ethanol found in red wine . However, alcohol is also a major risk factor for global disease and economic burden . Taken together, findings from the present study suggest that increasing intakes of particular flavonoid-rich foods, even in mid-life, may possibly have a real impact on risk of early mortality.
For the flavodiet score, we showed that an achievable three servings/day increase in intake of flavonoid-rich foods (for example one cup of tea, one serving of blueberries and one glass of red wine) was associated with an 8% lower risk of all-cause mortality in our multivariable-adjusted pooled analysis. This appeared to be driven by an increase in risk seen for participants with the greatest decreases in flavodiet score. To date, a wealth of evidence suggests that increased intakes of flavonoid-rich foods, including tea and berries, and several sub-classes of flavonoids, are inversely associated with biomarkers and risk of cardiovascular disease [3, 5, 30]. The most commonly investigated role for flavonoids in in vitro and animal studies relates to their potential cardioprotective role including regulation of nitric oxide synthase, COX-2 expression, and platelet aggregation . The lack of association between an increase in the flavodiet score and cardiovascular mortality was therefore surprising but may be explained by the diversity of foods, and their flavonoid composition, within the flavodiet score, not all of which may be cardioprotective. As well as for cardiovascular disease, chronic inflammation plays a key role in respiratory and neurodegenerative disease  and many flavonoid metabolites have been shown to interact with chronic inflammatory disease at a molecular level and modulate the response of key enzymes and cell-signalling cascades [6, 34, 35]. In population-based studies, a higher habitual intake of several flavonoids, including anthocyanins and flavonols, was associated with a lower level of inflammation [36, 37]. This may explain the observation in the present study that an increase of three servings/day of the flavodiet score was associated with a 13% lower risk of mortality from neurological causes and a 10% lower risk of respiratory mortality, although the latter did not reach statistical significance. As for all-cause mortality, this appeared to be driven by the higher risk of mortality from neurological causes seen for participants with the greatest decreases in flavodiet score. The limited data in human studies suggest that higher habitual flavonoid intakes are positively associated with lung function [38, 39] and we have recently shown that higher flavonoid intakes are inversely associated with incident COPD in current and former, but not in never, smokers . While we observed that a decrease in flavodiet score was associated with an increased risk of mortality in the present study, we observed no interaction with smoking status. There is more evidence for a protective role of flavonoids in the development of neurological diseases  with mechanisms including a reduction in reactive oxygen species and amyloid beta-protein production . Recent data also suggest that higher intake of flavonoids and flavonoid-rich foods are associated with a lower risk of Alzheimer’s, dementia, and Parkinson’s disease [43,44,45,46] but our data are the first to suggest that increasing intakes in mid-life can reduce risk of neurological death. Although we include a lag time of 2 years, we cannot discount the possibility that observed associations may be attributed to reverse causation, in particular, that a higher risk of neurological death may be linked to a decrease in intakes of flavonoid-rich foods. This is plausible because changes in diet due to neurologic degenerative diseases may occur many years before death and previous research reports that individuals with Alzheimer’s disease and dementia, which make up a large proportion of neurological deaths, have accelerated weight loss , malnutrition , and food preferences for sweet and salty foods . However, we also report a lower risk of neurological death with an increase in intakes of flavonoid-rich foods, a finding less susceptible to reverse causation when the comparator is those whose intakes did not change.
Strengths of this study include the prospective design, large sample size with long-term follow-up, repeated measures of dietary intake which allowed us to calculate the updated 8-year change in intakes, and detailed data on important risk factors and confounders. A potential limitation of the flavodiet score is the variability in the flavonoid composition between the seven foods and beverages. However, our analyses focus on flavonoid-rich foods and a flavodiet score, rather than specific flavonoid sub-classes and a weighted score, so that the public health relevance of the findings can be fully appreciated. While we have focussed on flavonoid-rich foods and beverages, we cannot rule out that the observed associations may be due to other constituents found in the whole food or to other uncaptured factors in the meal context. Furthermore, other flavonoid-bearing foods, such a dark chocolate and herbs, and detailed information on factors that influence a foods’ flavonoid content, such as plant variety, growing, storage, processing and cooking methods , were not captured in all FFQs. Although we adjusted for a wide range of possible confounders, there is still the possibility of residual confounding from additional unmeasured factors. However, given our detailed and updated adjustment for potential confounders, it is unlikely that these would account fully for the observed results. Furthermore, due to the observational nature of this study, we cannot conclude that observed associations are causal. Findings require replication in other populations; while the NHS and HPFS have high internal validity owing to the health knowledge and commitment to research of the participants , these two cohorts are not representative of the general population as they include only nurses or health professionals and participants were mostly Caucasian. Finally, because of the potential for reverse causation to influence these findings, further studies of these relationships are needed.
Altogether, findings from the present study suggest that not all flavonoid-rich foods are equal. Our novel data suggests that increasing intakes of specific flavonoid-rich foods, namely tea, blueberries, red wine and peppers, even in middle age, may reduce mortality risk.
Availability of data and materials
Data described in the article, code book, and analytic code will be made available upon request pending approval by the Channing Division of Network Medicine at Brigham and Women’s Hospital and Harvard Medical School. Further information including the procedures to obtain and access data from the Nurses’ Health Study and the Health Professionals Follow-Up Study is described at https://www.nurseshealthstudy.org/researchers (contact e-mail: email@example.com) and https://sites.sph.harvard.edu/hpfs/for-collaborators/.
Body mass index
Food frequency questionnaire
Health Professionals Follow-up Study
International Classification of Diseases
Nurses’ Health Study
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The authors would like to acknowledge the contribution to this study from central cancer registries supported through the Centers for Disease Control and Prevention’s National Program of Cancer Registries (NPCR) and/or the National Cancer Institute’s Surveillance, Epidemiology, and End Results (SEER) Program. Central registries may also be supported by state agencies, universities, and cancer centres. Participating central cancer registries include the following: Alabama, Alaska, Arizona, Arkansas, California, Colorado, Connecticut, Delaware, Florida, Georgia, Hawaii, Idaho, Indiana, Iowa, Kentucky, Louisiana, Massachusetts, Maine, Maryland, Michigan, Mississippi, Montana, Nebraska, Nevada, New Hampshire, New Jersey, New Mexico, New York, North Carolina, North Dakota, Ohio, Oklahoma, Oregon, Pennsylvania, Puerto Rico, Rhode Island, Seattle SEER Registry, South Carolina, Tennessee, Texas, Utah, Virginia, West Virginia, Wyoming.
This work was supported by the following grants from the National Institutes of Health (UM1 CA186107 and U01CA167552) and by funding from The US Highbush Blueberry Council (USHBC) with oversight from the USDA.
Ethics approval and consent to participate
The study protocol was approved by the Human Research Committee of Brigham and Women’s Hospital and the Harvard T.H. Chan School of Public Health. Completion and return of study questionnaires implied informed consent of the participants. IRB Protocol Number: NHS: 1999P011114 & HPFS: 10162.
Consent for publication
AC and EBR both act as advisors to the USHBC grant committee. All other authors declare no relevant conflicts of interest. The funders of the study had no role in study design, data collection, data analysis, data interpretation, or writing of the manuscript.
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Fig. S1. Flow chart of participants. Fig. S2. Time trends in mean intakes of flavonoid-rich foods in the Nurses’ Health Studyand Health Professionals Follow-Up Study. Table S1. Associations between all-cause mortality1 and 8-year change in intake of flavonoid-rich foods.
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Bondonno, N.P., Liu, Y.L., Zheng, Y. et al. Change in habitual intakes of flavonoid-rich foods and mortality in US males and females. BMC Med 21, 181 (2023). https://doi.org/10.1186/s12916-023-02873-z